Pathogens Fast Identification Service

Categories of Testing

Detection of pathogens by NGS [DNA pathogens]
Pathogens Fast Identification by NGS [DNA-based]

Purpose

Clinically, the number of infections caused by “fever of unknown origin” is gradually increasing, antibiotic resistance is evolving rapidly, and the mortality rate of derived bacteremia is extremely high, resulting in a rapid increase in the difficulty of treating infections. “Rapid detection” and “Precise identification” of infectious pathogens are critical to the treatment options and patient prognosis of “fever of unknown origin”. Traditional microbial diagnosis methods have limitations, and it is difficult to “quickly” and “accurately” identify infectious pathogens. PFI testing applies Metagenomic Next Generation Sequencing (mNGS) to compare the results with a database of 25,259 pathogens to accurately screen and identify pathogens. It is an important tool for differential diagnosis and treatment for fever and bacteremia of unknown origin and medication.

Leopard Gene applies mNGS to identify pathogens including 7,098 types of bacteria, DNA virus among 8,717 types of viruses, 618 types of parasites, 8,521 types of fungi , 305 types of archaea , antibiotics resistance genes and virulence factor analysis, and provide physicians with reference data for diagnosis.

Specification

Detection of pathogens by NGS [RNA pathogens]
Pathogens Fast Identification by NGS [RNA-based]

Purpose

Clinically, the number of infections caused by “fever of unknown origin” is gradually increasing, antibiotic resistance is evolving rapidly, and the mortality rate of derived bacteremia is extremely high, resulting in a rapid increase in the difficulty of treating infections. “Rapid detection” and “Precise identification” of infectious pathogens are critical to the treatment options and patient prognosis of “fever of unknown origin”. Traditional microbial diagnosis methods have limitations, and it is difficult to “quickly” and “accurately” identify infectious pathogens. PFI testing applies Metagenomic Next Generation Sequencing (mNGS) to compare the results with a database of 25,259 pathogens to accurately screen and identify pathogens. It is an important tool for differential diagnosis and treatment for fever and bacteremia of unknown origin and medication.

Since 2019 COVID-19 pandemic, a considerable number of cases has accumulated in many countries and it causes a dramatic change of lifestyles worldwide. Taiwan is well-known for its epidemic prevention system that keeps COVID-19 pandemic effectively controlled. Leopard Gene applies mNGS-based PFI testing to identify pathogenic virus species based on 8,717 virus databases. It also provides information of the existence of antibiotics resistance genes and the virulence factor analysis to assist clinical diagnosis.

Specification

Detection of pathogens by NGS (RNA virus)-in depth scan version
Pathogens Fast Identification by NGS [RNA-based PFI] (Enhanced)

Purpose

Leopard Gene Detection of pathogens by NGS (RNA virus)-in depth scan version applies Metagenomic Next Generation Sequencing (mNGS) technology to identify pathogenic RNA virus species based on 8,717 virus databases, antibiotics resistance genes and virulence factor analysis.

It becomes an important tool to identify pathogenic virus which causes unknown fever and sepsis as well as providing information for therapeutic strategies and medication.

Specification

Frequent asked questions

Many microorganisms that live in symbiosis with the human body include viruses, bacteria, molds and other human microbiota, which are dozens of times more than human cells, and are also widely distributed in the intestines, skin, oral cavity, or mucous membranes. The population and distribution of these microorganisms may change with the environment and cause diseases. Microbial imbalance often leads to the occurrence of diseases, quickly and correctly diagnose pathogenic bacteria and give treatment can effectively reduce the fatality rate.

With the vigorous development of molecular biotechnology, the clinical diagnosis of whether patient is microbial infection has been promoted from the traditional blood culture method to the next generation gene sequencing (NGS). NGS is a high-throughput new technology that can sequence a large amount of DNA at the same time, so as to provide a clinical basis for rapid and accurate diagnosis.

With the vigorous development of molecular biotechnology, the clinical diagnosis of whether a patient is microbial infection has been promoted from the traditional culture method to the next generation gene sequencing (NGS). NGS is a high-throughput new technology that can sequence a large amount of DNA at the same time, so as to provide a clinical basis for rapid and accurate diagnosis.

The outbreak of SARS nosocomial infection in Taipei City Hospital Heping branch was already in 2003. It took nearly 5 months from the discovery of SARS to the final determination of which pathogen was infected. At the present time now, viruses are getting smarter and smarter. Fortunately, the virus is evolving, and medical technology is also advancing at the same time. In particular, the ability to quickly identify new viruses was completely unimaginable in the past.
Take the new coronavirus as an example. The new coronavirus is still raging around the world, and new virus strains have been discovered in various places one after another ,arousing great attention from all over the world. After the Chinese government official announced its pathogen on January 9, 2020, within a week, it confirmed the full genetic sequence of the new coronavirus based on Next Generation Sequencing (NGS). This shows that NGS genetic testing can accelerate the identification of unknown pathogenic microorganisms.

The culture time of the traditional pathogenic microorganism detection method is as long as 6-8 weeks, and the sensitivity is not high. It is unable to quickly identify the type of pathogen and provide physicians with antibiotics and multi-drug resistance (MDRO) and other relevant information. The risk of nosocomial infection delays the patient’s recovery and discharge time.

LEOPARD Gene provides metagenomic next-generation genetic sequencing (NGS) genetic testing services. Next-generation gene sequencing (NGS) is a high-throughput new technology that can sequence a large amount of DNA at the same time. It can be applied to the detection of unknown infectious pathogens, the identification and analysis of novel microorganisms, the whole sequencing of microbial genomes, the typing of microorganisms, the investigation of infectious disease clusters, the analysis and comparison of drug resistance genes, and the tracking of changes in microbial flora and so on, to assist physicians of emergency room and intensive care quickly detect unknown pathogenic microorganisms.

Guard your health with the quality testing

Contact us